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Figure S2. Xenopus laevis Cst Developmental and Spatial Expression
(A) Developmental time course of X. laevis Cstα and Cstβ expression by RT-PCR of
egg lysate (maternal transcripts), whole embryo ranging from Stage 10 (gastrulation) to
Stage 42 (late tadpole), and adult heart. Ef1α is used as a positive control. (B-K) Whole
mount in situ hybridization of Stage 14 (neurula) to Stage 40 (tadpole) embryos using a
Cst-specific probe common to both Cstα and Cstβ. B,C are dorsal views with anterior to
the left. D-H, and J are lateral views with anterior to the left: hindbrain (hb), presomitic
mesoderm (psm), somites (s), heart primordium (hp), eye (e), otic vesicle (ot), nasal
placode (np), trigeminal ganglion (tg), facial placodes (p), heart (h), kidney (k), vascular
vitelline network (vvn). |
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Figure S3. Xenopus tropicalis Cst Spatial Expression
Whole mount in situ hybridization of Cst in X. tropicalis of Stage 14 (neurula) to Stage
40 (tadpole) embryos using a Cst-specific probe common to both Cstα and Cstβ. A is an
anterior view. B-F, I-J are lateral views with anterior to the left. G is a ventral view with
anterior to the top. H is a dorsal view with anterior to the left. hindbrain (hb), presomitic
mesoderm (psm), somites (s), heart primordium (hp), otic vesicle (ot), nasal placode
(np), facial placodes (p), heart (h), kidney (k), vascular vitelline network (vvn). |
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casz1 (castor zinc finger 1) gene expression in Xenopus tropicalis embryo, assayed via in situ hybridization, NF stage 36, lateral view, dorsal up, anterior left. |
