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Fig. 3. Expression of CCT-y in neural crest and neural tube in Xenopus
embryos. Whole embryos were stained by whole mount in situ hybridization
(see Experimental Procedures) using an RNA probe synthesized
from the full-length cDNA. Embryos were sectioned in the
horizontal plane. A: Stage 23 embryo shows expression in the neural
ectoderm (n), eye anlage (e), and migrating cranial neural crest (arrows).
B: Expression in the visceral arches of a stage 28 embryo. C: A more
dorsal section of the embryo in B shows hybridization in the neural tube
(n). eye vesicle (e), and neural crest (arrow). D: Expression is also seen
in the visceral arches of the stage 35 embryo. ph, pharynx; cg, cement
gland. |
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Fig. 2. Expression of CCTy in whole mount in situ preparations of
stage 30 Xenopus embryos. Hybridization with an antisense RNA probe
(top) identifies transcripts in the neural tube (arrowhead), pharyngeal
arches (thin arrow), and dorsolateral mesoderm (thick arrow). A sense
RNA probe (bottom) shows no specific cellular staining. There is faint
staining in the pharyngeal cavity c); histological analysis confirms that
this is not associated with cells (not shown). |
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cct3 ( chaperonin containing TCP1, subunit 3 (gamma)) gene expression in Xenopus laevis embryo, NF Stage 30, as assayed by in situ hybridization. Lateral view: Dorsal up, Anterior left |
