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F1c. 3. Spatial distribution of Xcad-3 transcripts in the early Xenopus embryo. Localization of Xcad-3 transcripts was determined by wholemount
in situ hybridization using a digoxygenin·labeled antisense RNA probe (Fig. 1C, probe b). Embryos inA-Care albino embryos and those
shown in D-F are pigmented embryos which have been cleared in benzyl alcohol:benzyl benzoate. (A) St.nge 8 embryo. A faint stain of Xccul-3,
barely above backgrouud levels, is detected. (B) Stage 9 embryos. At stage 9, the animal cap (An) distinctly stains for Xcad-S expression while
there is no visible stain in the vegetal hemisphere (Vg). The unlabeled embryos are posi tioned with their vegetal side up. (C) Stage 10 embryos.
Xcad-3 is expressed in a band at the mnrginal zone. The uppermost embryo is tilted such that the ma..ginal zone is perpendicular to the page and
a distinct band of stain tan be seen encircling the embryo. The embryo at the lower left is shown in a vegetal view and the dorsal blastopore lip is
indicated. The embryo to the riiht is viewed from the animal hemisphere. (D) Stage 12 embryo. The band of Xcad-a expression encircles the
blastopore lip. The embryo is oriented with the dorsal side up. (E) Stage 15 embryo. Xcad-3 is expressed in the dorsal and ventral posterior
mesoderm and in the neural fold region. The embryo is oriented with anterior to the right and dorsal to the top of the page. (F) Stage 22
embryos. Xcad-3 is expressed primarily in the posterior neural tube. The embryos are oriented with anterior to the right and dorsal to the top of
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