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Fig. 7. Localization of xC/EBP mRNA by in situ hybridization during early metamorphosis. Bright-field (a, c, e and g) and dark-field (b, d, f and
h) images of localization of xC/EBP mRNA in different tissues of stages 55/56 and 57/58 Xenopus tadpoles. Sagittal sections were hybridized
with antisense or sense 35S-labelled xC/EBP cRNA probe (1 x 105 cpm/tzl) and subsequently treated with RNase A as described by Wilkinson
and Green (1990). Sense probe gave virtually no hybridization in the kidney and fat body but low background was observed in the liver. (a, b)
Liver of stage 55/56 tadpole. (c, d) Liver of stage 57/58 tadpole. (e, f) Kidney and fat body (Fb) of stage 55/56 tadpole. (g, h) Kidney of stage
57/58 tadpole. Note that pigmented layers (Pi) appear as bright zones in dark-field images, also seen with sense probe controls. Autoradiographs
exposed for two weeks. The bars represent 100/xm |
