| |
Figure 4. Distribution of CB1 mRNA labeling in the different layers of the olfactory bulb and anterior olfactory nucleus (A,B, longitudinal parasagittal sections). Some lightly stained periglomerular cells are shown in the glomerular layer of the olfactory bulb; some positive cell bodies are seen in the anterior olfactory nucleus. Intensely labeled cell bodies occur in the mitral and tufted cell layer as well in the granule cell layer (A). Positive cell bodies of different sizes are shown in more detail within the layer containing both mitral (arrows) and tufted cells (arrowhead) (B). A transverse section of the prosencephalon (C) shows abundant positive cell bodies distributed throughout the pallium and subpallial sites of the telencephalon such as amygdaloid nucleus and striatum. An intense staining is also observed in the anterior preoptic nucleus. Higher magnifications of CB1 mRNA-positive neurons in the medial pallium (D), in the pars medialis of the amygdaloid nucleus (E), and in the dorsal pallium (F). A: anterior; AON: anterior olfactory nucleus; Apl: amygdala pars lateralis; Apm: amygdala pars medialis; Dp: dorsal pallium; gcl: granule cell layer; gl: glomerular layer; ml: mitral cell layer; Mp: medial pallium; P: posterior; Poa: anterior preoptic nucleus; Str: striatum. Scale bars = 80 μm in A, 40 μm in B, 450 μm in C, 140 μm in D, 80 μm in E and F. |
