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Fig 4. Type I and type II collagen expression patterns in the Xenopus limb bud.(A-C) The st. 52 Xenopus limb bud. (A) HE and Alcian blue staining. (B) Type I collagen expression. (C) Type II collagen expression. (D-F) The distal part of the st. 54 Xenopus limb bud. (D) HE and Alcian blue staining. (E) Type I collagen expression. (F) Type II collagen expression. (G-I) The distal part of the st. 56 Xenopus limb bud. (G) HE and Alcian blue staining. (H) Type I collagen expression. (I) Type II collagen expression. A-C are shown at same magnification. D-I are at same magnification. Scale bars in A, B insert, D are 500 μm, 200 μm, 100 μm, respectively. Arrowheads indicate presumed cartilaginous regions. |