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Fig. 3. Spatial expression pattern of Xenopus COL1A1 shown by whole-mount in situ hybridization. (A) Stage 25 embryo. Weak expres- sion of Xenopus COL1A1 was detected in the posterior dorsal region. (B) Stage 28 embryo. Expression of Xenopus COL1A1 gradu- ally spread to the anterior region. (C) Stage 30 embryo. Expression of Xenopus COL1A1 was extended to the head region. (D) Transverse sections of the stage 30 embryo at the levels indicated by the white bars in (C). (D,F) A section at the head region (bar 1). The transcripts were detected in the inner layer of epidermis. Note that the signals extended to the ventral region (arrowheads). (E,G) A section at the trunk region (bar 2). Intense staining was detected in the inner layer of the dorsal epidermis. Less intense sig- nals were detected in the outer layer of the dorsal epidermis and dermatome. COL1A1 was not detected in the ventral epidermis. Bars, 1 mm (A), 150 μm (D), 75 μm (G) |
