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Figure 2. Comparison between st58 tibia and spike cartilage. Hematoxylin-eosin and alcian blue staining of the section of st58 tibia. A,B: The cartilage was well differentiated and the obvious boundary between the cartilage and other tissue was formed in the diaphysis. Some flat cells forming the perichondrium were observed (B, arrows). C–F: Double-immunolabelling of st58 tibia with antibodies specific for type I and type II collagen. C,D: In the diaphysis, immunolocalization of type I collagen in the outer layer of the perichondrium (C, arrows) and type II collagen in the inner cellular layer of the perichondrium and cartilage matrix was visualized, using rhodamine- and fluorescein isothiocyanate-conjugated secondary antibodies, respectively. E,F: In the epiphysis, many chondrogenic cells exhibited immunoreactivities for both type I and type II collagen. G,H: Morphology of the spike cartilage. Marginal region of the spike cartilage was slightly stained with alcian blue (H, arrows). I,J: Immunoreactivities for type I and type II collagen. Obviously separated signals of type I and type II collagen were not observed in the marginal region of cartilage (solid line). Nuclei were stained with DAPI (C–F, I, J). Scale bar = 50 μm. |
