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Fig 5. Type I and Type II collagen expression patterns in Xenopus stage 52 and stage 56 limb bud blastemas.(A-C) On day 10 following zeugopod amputation at st. 52 limb bud. (A) HE and Alcian blue staining. (B) Type I collagen expression. Type I collagen expression was weak in the distal region. (C) Type II collagen expression. (D-F) On day 10 following zeugopod amputation at st. 56 limb bud. (D) HE and Alcian blue staining. (E) Type I collagen expression. Type I collagen expression was observed throughout the entire mesenchymal region. (F) Type II collagen expression. A-C are shown at the same magnification. D-F are shown at the same magnification. A’-F’ are higher magnification images of A-F, respectively. Scale bars in A, D, C’, D’, are 200 μm, 500 μm, 1 mm, 250 μm, respectively. Black bars indicate amputated planes. Arrowheads indicate estimated cartilage forming areas. |
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Fig 4. Type I and type II collagen expression patterns in the Xenopus limb bud.(A-C) The st. 52 Xenopus limb bud. (A) HE and Alcian blue staining. (B) Type I collagen expression. (C) Type II collagen expression. (D-F) The distal part of the st. 54 Xenopus limb bud. (D) HE and Alcian blue staining. (E) Type I collagen expression. (F) Type II collagen expression. (G-I) The distal part of the st. 56 Xenopus limb bud. (G) HE and Alcian blue staining. (H) Type I collagen expression. (I) Type II collagen expression. A-C are shown at same magnification. D-I are at same magnification. Scale bars in A, B insert, D are 500 μm, 200 μm, 100 μm, respectively. Arrowheads indicate presumed cartilaginous regions. |
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Fig 6. Type I and Type II collagen expression patterns in the Xenopus blastema.(A-C) The Xenopus blastema at 10 days postamputation. Insert indicates Proximal-Distal axis sections. (A) HE and Alcian blue staining. (B) Type I collagen expression. (C) Type II collagen expression. (D-F) The Xenopus blastema at 20 days postamputation. (D) HE and Alcian blue staining. (E) Type I collagen expression. (F) Type II collagen expression. (G-I) The Xenopus blastema at 30 days postamputation. (G) HE and Alcian blue staining. (H) Type I collagen expression. (I) Type II collagen expression. A-I are shown at the same magnification. A’, B’ and C’ are higher magnification images of A, B and C, respectively. Scale bars in A are 500 μm. Scale bar in A’ is 200 μm. |
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Fig 8. Type I and type II collagen expression patterns in Xenopus ALM blastemas.(A-H) The Xenopus ALM blastema. (A-D) The Xenopus ALM blastema at 10 days postoperation. (A) HE and Alcian blue staining. (B) Type I collagen expression. (C) Type II collagen expression. (E-H) The Xenopus ALM with deep wound blastema at 20 days postoperation. (E) HE and Alcian blue staining. (F) Type I collagen expression. (G) Type II collagen expression. (I-T) The Xenopus ALM with deep wound blastema. (I-L) The Xenopus ALM with deep wound blastema at 10 days postoperation. (I) HE and Alcian blue staining. (J) Type I collagen expression. (K) Type II collagen expression. (M-P) The Xenopus ALM with deep wound blastema at 20 days postoperation. (M) HE and Alcian blue staining. (N) Type I collagen expression. (O) Type II collagen expression. (Q-T) The Xenopus ALM with deep wound blastema at 30 days postoperation. (Q) HE and Alcian blue staining. (R) Type I collagen expression. (S) Type II collagen expression. (D, H, L, P, T) Control of in situ hybridization experiments. Sense probe of type I collagen. All are shown at the same magnification. Scale bar is 500 μm. Black arrowheads indicate wound line. White arrowheads indicate bone cracked region. |
