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Fig. 3. Expression of Crabp2 during organogenesis. In situ hybridisation of Crabp2, dark blue stain indicates expression. (A) whole mount embryos with anterior to the left and dorsal uppermost. (A) Expression at stage 28. (B) Expression at stage 30. (C) Expression at stage 34. Red arrows indicate positions of transverse sections in panels D. (C Detail of head region of embryo pictured in panel C. (D) Transverse sections through stage 34 embryo. Scale bars are 100 lm. (D) Staining in head mesenchyme ventral to olfactory placodes, at the level of the telencephalon. (E) Staining in branchial arches and dorsal retina at eye level. (F) Staining in the hindbrain is strongest at the lumenal surfaces. (G) Sagittal section though stage 34 embryo showing staining in branchial arches, head mesenchyme, midbrain/hindbrain boundary region and posterior hindbrain. Scale bar is 100 lm. Abbreviations: ba, branchial arches; dr, dorsal retina; hb, hindbrain; hm, head mesenchyme; le, lens; m/h, midbrain hindbrain boundary; no, notochord; op, olfactory placode; ov, otic vesicle; pd, pronephritic duct; s, somite; tb, tailbud; te, telencephalon. |
