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Fig. 1.
RT-PCR analysis of CRF related mRNAs in brain areas of X. laevis. 2% agarose gels showing RT-PCR analysis of CRF related mRNA expression in X. laevis brain tissue. Two micrograms of total RNA from each brain area was reversed transcribed before PCR amplification and separation on a 2% gel. Relative expression of the ribosomal protein L8 (RPL8) is shown as a housekeeping control for the integrity of the total RNA. Primer sequences based upon Boorse and Denver (2006). Tel, telencephalon; H/T, hypothalamus, thalamus; OT, optic tectum; BS, brainstem. |
