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Fig. 2. Anti-Xdazl immunocytochemistry of the testis (A,B), ovary (C,D), early stage embryos (E,F) and the genital ridges in embryos (G,H). (A) Xdazl
expressions (appearing in red) in spermatogenic cells (PG, primary spermatogonium; SG, secondary spermatogonium; C, spermatocyte; T, spermatid, M,
mature spermatozoon). (B) Control staining without primary antibody. (C) Xdazl expression in oogonia (G) but not in the early oocytes (C). (D) Presence of
Xdazl in the perinuclear cytoplasm of growing oocytes but absence in germinal vesicle (GV). (E) Xdazl expression in the stage 5 (16-cell stage) embryo. Xdazl
is distributed throughout the embryo. (F) The vegetal pole of the stage 4 (8-cell stage) embryo showing no speci localization of Xdazl to the germ plasm
(indicated by an arrow). (G) Xdazl expression in primordial germ cells (indicated by arrowheads) in the stage 47 embryo. (H) Anti-Xdazl-positive primordial
germ cells (arrowheads) in the stage 52 embryo. Scale, 500 mm (E); 100 mm (A,B,D,F); 50 mm (C,G,H). |
