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Fig. 1. Ednrb2 and melanoblast marker gene expression patterns in Xenopus embryos by WISH. A,B: Mitf expression is detected (A), but there is no Ednrb2 expression in a stage-19 embryo. C: Ednrb2 expression (arrowheads) is first detected in the NT at stage 21. D–F: Weak Dct (D) and strong Ednrb2 (E,F) expression is detected at stage 25. G–I: Dct (G) and Ednrb2 (H,I) expression is detected in the NT and only Ednrb2 is expressed in the head region at stage 28 (red arrowheads). J–R: The expression pattern of Ednrb2 (K,O,R) resembles that of Dct (J,M,P) except in the RPE (Dct only). Blue arrowheads indicate expression in the epiphysis. Note that after stage 35, melanophores are pigmented, but because the embryos are bleached in these embryos, the melanins are not visible. Insets are magnified images in the squares (scale bar ¼ 100 mm) and show dendritic pigmented melanophores expressing both Dct (P) and Ednrb2 (R). F, I, L, O, and R are lateral views of E, H, K, N, and Q, respectively. Heads are on the right side of each picture. Scale bar ¼ 1 mm. |