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Fig 6. Dsel is expressed maternally and in the eye, brain and cranial neural crest.
Embryos after whole-mount in situ hybridization are shown in animal view (A), lateral view (B,E,E’,I,I’), hemi-sectioned (C) and transversally sectioned (F-H). (A) 4-cell stage embryo. (B,C) Blastula embryos. The bold line indicates the level of section in C. (D) qPCR analysis at stage 9. Note ubiquitous expression of Dsel in the animal cap, marginal zone and vegetal region. (E-I’) Tailbud embryos. The bold lines indicate the level of sections in F-H. Note Dsel expression in the eye, interneurons of the mid- and hindbrain (arrows), cranial neural crest (indented arrowheads) and bilateral trigeminal ganglia. an, animal cap; ey, eye; hb, hindbrain; ma, marginal zone; mb, midbrain; V, trigeminal ganglion, vg, vegetal region. Scale bars are 500 μm. |
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Fig 7. Dsel is expressed in differentiated neurons, cranial sensory ganglia and the spinal cord.
Embryos are shown in lateral view (A-B’,K,K’) and transversal vibratome sections (C-J). (A,A’) Embryo at stage 33. The bold lines indicate the level of sections in C-J. Magnification in A’ depicts Dsel mRNA in cranial sensory ganglia: V, trigeminal ganglion; VII, geniculate ganglion; IX, petrosal ganglion; X, nodose ganglion. (B,B’) Sibling embryo depicting N-tubulin expression in the central nervous system (stars) and cranial ganglia. (C-J) Dsel is expressed in the adenohypophysis and distinct neurons (arrows) of the forebrain, midbrain, hindbrain and spinal cord. The indented arrowhead labels signals in migrating cranial neural crest cells. (K,K’) Embryo at stage 34. The indented arrowhead labels robust Dsel transcripts in post-migratory cranial neural crest cells. ad, adenohypophysis; df, dorsal fin; ea, ear; ey, eye; fb, forebrain; hb, hindbrain; ist, isthmus; IX, petrosal ganglion; mb, midbrain; no, notochord; pm, sp, spinal cord; V, trigeminal ganglion; VII, geniculate ganglion; IX, petrosal ganglion; X, nodose ganglion. Scale bars are 1 mm (A,B,K,K’) and 200 μm (A’,B’,C-J). |
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dsel (dermatan sulfate epimerase like) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 33 & 34, close-up of neurogenic placode region, lateral view, anterior left, dorsal up. |
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dsel (dermatan sulfate epimerase like) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 33 &34, lateral view, anterior left, dorsal up. |
