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Figure 4. Expression of HNF1β, ZFP36L1, and E4F1 in Xenopus embryos. (a) Whole-mount in situ hybridization of HNF1β, E4F1, and ZFP36L1 in Xenopus neurula and tailbud embryos using antisense probes. HNF1β is predominantly expressed in the pronephros anlage in neurula stage (arrow) as well as in the anlage of the pronephric tubule (arrow) and duct (arrow head) of the tailbud stage. Arrows and arrow heads mark the pronephric tubule and duct anlage, respectively, observed in tailbud embryos probed with E4F1 or ZFP36L1. ZFP36L1 is the Xenopus ortholog C3H-2. (b) Whole-mount in situ hybridizations with the corresponding sense probes. |
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e4f1 ( E4F transcription factor 1 ) gene expression in X.laevis embryo, NF stage 27/28, assayed via in situ hybridization, anterior right, dorsal up. |