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Fig. 1. In situ hybridization of an EF-1aS probe to sections of tadpole gonads. Section of stage 55 gonad (premetamorphosis) hybridized with sense (A) or anti-sense probe (B). Gonia (arrows) are distinctly labeled. Section of a testis (C) and of an ovary (D) at stage 62 (metamorphosis) hybridized with anti-sense probe. (E and F) Sections of stage 66 ovary hybridized with anti-sense probe. The hybridization signal in germ cells (arrows) drops noticeably between stage 55 and stage 66. A-C and E. darkfield illumination. 0 and F. bright-field illumination. Scale bars. 50 um |
