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Fig. 2.
Panel A: RT-PCR of eEF1A1 (top panel) and eEF1A2 (bottom panel) in RNA from a range of tissues taken from adult X. laevis. Li = liver, Lu = lung, S = spleen, O = oocytes, G = gall bladder, B = brain, H = heart, M = muscle (two independent tissue samples). No-RT controls were carried out and were negative for all tissues for both genes (data not shown). Panel B: Western blots of eEF1A1 (top) and eEF1A2 (bottom). Gels were run in duplicate using the samples of same protein extract at the same time. Og = optic ganglion, B = brain, Sc = spinal cord, M = muscle, Li = liver, G = gall bladder, Lu = lung, K = kidney, S = spleen. Panel C: Immunohistochemistry for eEF1A2 on spinal cord and cardiac muscle from X. laevis, together with negative controls. eEF1A2 shows widespread expression in cardiac muscle but is expressed in only in neuronal cells in spinal cord.
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