| |
Figure 4. ephrinB1 Rescues the cFGFR Retinal Phenotype(A) Anterior views at stage 16 show control expression domains of pax6, ephrinB1, and fgfr2. Asterisks indicate the anterior tip of the neural groove, allowing an anatomical comparison of the domains.(B) Blastomeres that contribute large numbers (D1.1.1, top) or very small numbers (V1.2.1, bottom) of cells to the retina were injected with β-gal mRNA, and the location of anterior descendants (red) compared to the expression domains of pax6, ephrinB1, and fgfr2 (blue patches, arrows). Top, frontal views; bottom, pax6 is frontal; ephrinB1 and fgfr2 are side views.(C) Animal poles at stage 12 showing D1.1.1 clone (red) coexpressing cFGFR2 and ephrinB1.(D) Anterior view at stage 16 showing D1.1.1 clone (red) coexpressing cFGFR2 and ephrinB1.(E) Coexpression of ephrinB1 rescues the cFGFR2 repression of D1.1.1 contribution to the retina in a dose-dependent manner. ephrinB1-60AA does not rescue.(F) Coexpression of ephrinB1 with cFGFR2 allows D1.1.1 progeny (green) to populate the retina. |
