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Figure 1. Nucleolin localization. Indirect immunofluorescence
microscopy on isolated liver nuclei from Xenopus using
the purified antiserum raised against CHO nucleolin (A) and
corresponding phase contrast (B). Nucleoli are stained positively.
Erythrocytes are not stained (e). Frozen section of
Xenopus ovary (C) showing part of an oocyte nucleus (N) with
numerous nucleoli after incubation with anti-nucleolin antibody.
The amplified nucleoli (nu) at this stage (st. IV) are
strongly fluorescent. (D) Phase contrast: the nucleus is circled.
Bar, 50 ~m. |