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Fig. 3. Eomes expression, by in situ hybridization to a dissected brain from a stage 51 tadpole, is seen most strongly in the olfactory region of the anterior forebrain. (A) dissected brain; (B) ventral and dorsal view of telencephalon, dorsal view of hindbrain; (C) vertical section to one side of midline of Eomes- stained brain; (D) cresyl violet stain of section shown in (C). Abbreviations: aop, area olfactoria primitiva (arrows); e, epiphysis; c, Cb cerebellum; VA, ventral anterior; DA, dorsal anterior. Surface pigment cells appear as irregular black dots; Eomes staining is purple. |