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Fig. 8. Whole-mount in situ hybridization analysis of G50 expression
in neurula and early tailbud stage Xenopus embryos. A: Lateral view of a
stage 17 embryo stained for G50 mRNA expression. Transcripts are
detected in two bilateral stripes of cells in the head region (arrowheads),
and diffusely in the posterior end of the embryo. 6: Dorsal view of A at
higher magnification showing in greater detail the localization of G50
mRNAs in premigratory (arrow) and early migratory neural crest cell populations
(arrowhead). C: Lateral view of a stage 21 embryo stained for
Xenopus En-2 to localize the midbrain-hindbrain junction. D: Lateral view
of a stage 21 embryo stained for G50. Expression of G50 mRNAs is seen
in a stripe of cells at the height of the presumptive rhombomere 3, and in
neural crest of the hyoid arch (h). E: Lateral view of a stage 21 embryo
stained for Xenopus Krox-20. Two stripes of cells in the hindbrain corresponding
to the presumptive rhombomeres 3 and 5, and neural crest of
anterior branchial arch (ab) are expressing XKroxPO mRNAs. F Lateral
view of a stage 21 embryo stained simultaneously for G50 and XKroxPO.
Labeling of stained tissues is as in D and E, respectively. The intensity of
the background stain varies with respect to the length of incubation of the
embryos in the chromogenic reaction. Embryos in C and E were developed
for 1 hr, while the other embryos were incubated 16 hr to obtain
optimal staining. The stars in C and F indicate staining artifacts. Scale
bars: A = 160 pm; 0 = 80 pm; C F = 144 pm. |
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epha2 (EPH receptor A2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 21, lateral view, anterior left, dorsal up. |
