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Fig. 1. Expression of Xenopus EphB4 in the
embryonic vasculature. Transcripts for EphB4
(A,B), Msr (C), EphB1 (D), EphB2 (E), and
EphB3 (F) were detected by whole-mount in
situ hybridization of stage 34 (A,C-F) and 36
(B) embryos. Lateral views are shown with
anterior to the left. (A) Expression of EphB4 in
the pronephric sinus (arrow) and the posterior
cardinal vein (arrowheads) of the embryonic
trunk. (B) Close-up view of the trunk region to
illustrate EphB4 expression in the intersomitic
veins (arrows), the vascular vitelline vein
network (arrowhead), the pronephric sinus (ps)
and the posterior cardinal vein (pcv).
(C) Staining with the vascular marker Msr
visualizes the vascular vitelline network (vvn),
the common cardinal vein (ccv), pronephric
sinus (ps), the posterior cardinal vein (pcv), and
the intersomitic veins (iv). (D-F) EphB1,
EphB2 and EphB3 transcripts are prominently
detected in the head (fb, forebrain; mb,
midbrain; e, eye; ov, otic vesicle; hm, head
mesenchyme), in specific visceral arches (v1,
v4), the cement gland (cg), and the pronephric
region (pr), but not in intersomitic veins. Scale
bars: 500 mm (A,C-D); 200 mm (B). |
