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Fig. 3. Ets-1 transcript localization in Xenopus laevis gastrula (stage 101121. (A) Whole-mount In situ hybridizarson with antisense and sense probes (left and right embryo respectively). Ers-l transcripts dre observed as a Clfcumblastoporal collar (blue staining in the left embryo), whereas no signa/is observed with the sense probe. (BI Sect/on of astage 101/1 embryo showing the dorso-blastoporaf lip (red arrowhead) and rhe blastocoel (BL). The ets-l stainmg is mamly observed in the upper eplthebal layer of the marginal zone, and to a lesser extent In the deep marginal zone. However, cells rolling around the dorsoblastoporal lip do not seem to be labeled. In agreement with the absence of staining in a narrow cordon immediately adjacent fa the blastoporal lip in the left embryo(A) Bar, 100um. |
