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Figure 2. Expression of Eya genes. A,B: Eya1 is diffusely expressed throughout the ectoderm (ecto) of the animal (an) cap of the blastula (A) and the gastrula (B; side views). veg, vegetal pole; bl, blastopore lip; endo, endoderm. C–E: Eya3 is detected in the animal blastomeres of the 32-cell embryo (C), the animal cap of the blastula (D), and enhanced in the dorsal ectoderm of the late gastrula (E; side views). F: At neural plate stages, Eya3 is expressed throughout the neural plate (np) and preplacodal ectoderm (PPE), but not the cement gland (cg; anterolateral view). G–O: Eya2 expression. G: Eya2 is diffusely expressed throughout the neural plate and PPE (Anterior view). H: Upon neural tube closure, Eya2 is expressed in the adeno-hypophyseal (AH), olfactory (olf), trigeminal (Vp), and dorsolateral (dlp) placodes. I: It also is detected in the caudal neural tube (nt) and somites (so) (H, anterior view; I, dorsal view). J–L: At tail bud (J) and larval (K,L) stages, Eya2 is strongly expressed in the olfactory (olf), otic (oto), and various other placodes (pl) and their cranial ganglion derivatives (crg). Note that the lens placode (L) does not express Eya2. There is additional expression in the nephric mesoderm (ne) and hypaxial muscle precursors (hyp; J, K, side views; L, anterior view). M–O: In transverse sections at the levels of the diencephalon (M), otocyst (N), and caudal hindbrain (O), Eya2 expression is notable in the cranial ganglia (profundal, Pg; maxillo-mandibular branch of the trigeminal, Vg; VIIIg, IXg), otocyst, and somitic and nephric mesoderm. |
