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Figure 1. Expression of Fam183b. (A) Phylogram of vertebrate Fam183 genes rooted on human FAM183A.
Sequences used for alignment and phylogenetic analysis: human, HGNC-34347 and HGNC-34511; mouse,
Q5NC57; chicken, F1P3Y5; Xen. tropicalis, XP_004914015; Xen. laevis, XP_018113781; zebrafsh, ZDBGENE-111103-1.
(B) WISH of E7.75 wild type (wt) (a) and NotoGfp/Gfp (b) mutant embryos shows NOTOdependent
expression of Fam183b in the LRO. (C) Analysis of Fam183b expression by RT-PCR of RNA from
adult organs, as indicated. Full size gel is shown in Supplementary Figure S1. (D) SISH analysis of adult tissues,
as indicated. Boxed areas in a–f outline regions shown at higher magnifcation in a’–f ’. Arrows point to sites of
expression. FT: fallopian tube; CP: choroid plexus; PRL: photoreceptor layer; INL: inner nuclear layer; GCL:
ganglion cell layer. (E) Expression of fam183a in Xenopus laevis. Fam183a mRNA was found at stage 20 in
the foor plate (FP) and LRO (GRP; a,a’); at stage 34 in MCCs and nephrostomes (b); and at stage 45 (c) in the
sub-commissural organ (SCO), the zona limitans intrathalamica (ZLI) and the foor plate of the brain (c’),
in the dorsal lining of the branchial chamber (inset in c and c”), and the stomach (c”’). (F) Fam183a is a foxj1
target gene. Embryos were unilaterally injected on the lef side with a foxj1 mRNA and analysed for fam183a
expression. foxj1, injected side. Scale bars: Da–f=500μm, a’–f ’=100μm. |
