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Fig. 3. Expression of Fez in relationship to other anterior genes. Double in situ hybridization at stage 17 detecting Fez (magenta) and mid/hindbrain marker, en2 (turquoise) (A); Fez (magenta) and cement gland marker, Xag1 (dark purple) (D); and Fez (dark blue) and forebrain maker, XBF-1 (turquoise) (G). (B,E,H) Sagittal sections (B,E) and a horizontal section (H) of embryos shown in (A,D,G), respectively. Note the co-expression of Fez and XBF-1 in the prosencephalic region (G,H). (C,F,I) Double in situ hybridization at stage 27 displaying Fez (magenta)+en2 (turquoise) (C); Fez (turquoise)+Xag1 (magenta) (F); and Fez (purple)+XBF-1 (turquoise) (I). Note the expression of XBF-1 in the branchial arch where Fez expression is absent (I). |
