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FIG. 1. Endogenous and exogenous expression of FGF-2. (A-D) Localization of FGF-2 mRNA by in situ hybridization in 12~m transverse
sections through Xenopus eye prirnordia at different developmental stages: (A) stage 24, (B) stage 28, (C) stage 33/34, (D) sense control. [E---G)
Co-injection of CS2-GFP and CS2-FGI~2 into blastomeres at the embryonic 16~cell stage results in most transgene-positive cells expressing
both FGF-Z and GFP. (E) GFP (green) expression in the retina. (F) hnmunolabeling of FGF-2 transgene-expressing cells (red) using a rabbit
polyctonal antibody against FGF~2 (R&D Systems). (G) Coexpression (yellow) of GFP and FGF-2. L, lens; E, epidermis; PE, pigment
epithelium; D, dorsal; V, ventral; RGCL, retinal ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer; onh, optic nerve
head. Orientation arrows in D and E refer to A-D and E G, respectively. Scale bar in A is 50 gm for all. |
