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Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
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fgfr1 | xenopus | dorsal marginal zone [+] |
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Experiment details for fgfr1
Competition for ligands between FGFR1 and FGFR4 regulates Xenopus neural development.Competition for ligands between FGFR1 and FGFR4 regulates Xenopus neural development.
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Fig. 1. Differential expression of XFGFR1 and XFGFR4 on the dorsal side of embryos. (A) RT-PCR analysis of XFGFR1 and XFGFR4 mRNAs in the prospective anterior region (I) and posterior region (II) (each 0.45 mm wide and 0.2 mm long) excised from the dorsal side of stage-10 embryos. W: whole embryos. (B,C) XFGFR1 (B) and XFGFR4 (C) expression in stage-11 embryos. Arrowheads indicate the dorsal blastopore groove. (D) A stage-11 embryo was sagitally bisected through the dorsal blastopore groove (arrowheads), and the halves were hybridized for XFGFR1 (left) or XFGFR4 (right). Dotted lines indicate the ectoderm/ mesoendoderm boundary. (E) A stage-11 embryo was sagitally bisected, and the halves were hybridized for XFGFR4 (left) or XHR1 (right). Before bisection, the dorsal side was flattened by gentle pressure to compare the distances between the posterior limits of the expression regions and the blastopore groove (arrowheads). |