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Figure 5. Immunofluorescence analysis of the extracellular matrix and basement membrane surrounding the Xenopus foregut and lung buds. A–R: Confocal immunostaining of transverse sections through the NF35 Xenopus foregut at three positions along the anterior–posterior axis showing protein expression of (A–C) Fibronectin, (D–F′) Heparin sulfate proteoglycans, (G–I) FoxF1, (J–L) Laminin alpha-1, and (M–O) Chondroitin sulfate proteoglycans. Abbreviations: bm, basement membrane; dfg, dorsal foregut; lb, lung bud; lpm, lateral plate mesoderm; vfg, ventral foregut. Scale bar = 0.1 mm.Download figure to PowerPoint |