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Figure 4. Transverse Sections of Embryos Stained by Whole-Mount
In Situ Hybridization for XFS-319 RNA Distribution
(A) Transverse section of a late gastrula (stage 11.5). Animal pole is
at the top, dorsal to the left, and ventral to the right. XFS-319 RNA is
exclusively localized to the dorsal side of the embryo. RNA staining
is perinuclear and restricted to cells in the deep layer, prechordal
mesoderm, and anterior chordal mesoderm. The arrow marks the yolk
plug.
(B) Transverse section of an early neurula (stage 14) embryo showing
the localization of XFS RNA in the notochord, just underneath the
open neural plate, and the cells of the hypochord.
(C) Transverse section through the forebrain of a swimming tadpole,
showing that XFS RNA is localized as three dorsoventral stripes in
the cells of the ventricular zone (arrows). Staining is absent from
the floor plate and roof plate. For both (B) and (C), dorsal side is at
the top. |