|
Display additional annotations [+]
Gene |
Clone |
Species |
Stages |
Anatomy |
gli2
|
|
laevis
|
NF stage 41
|
retina
,
brain
,
forebrain
,
hindbrain
,
midbrain-hindbrain boundary
,
[+]
|
gli1
|
|
laevis
|
NF stage 28
|
brain
,
hindbrain
,
tail bud
,
chordoneural hinge
|
gli1
|
|
laevis
|
NF stage 41
|
brain
,
stomodeal-hypophyseal primordium
,
head region
,
pharyngeal arch
,
eye
|
ptch1
|
|
laevis
|
NF stage 41
|
retina
,
brain
,
head region
,
eye
,
ganglion
|
ptch2
|
|
laevis
|
NF stage 41
|
retina
,
brain
,
midbrain
,
hindbrain
,
stomodeal-hypophyseal primordium
,
[+]
|
|
|
Fig. 6. Cyclopamine treatment blocks Hh signalling in Xenopus. Whole-mount in situ hybridisation on embryos incubated in cyclopamine solution from stage 20 onwards (B,D,F,H,J,L) or control embryos (A,C,E,G,I,K). The probes are indicated for each panel. (C-L) Stage 41 embryos; (A,B) stage 30 embryos. Cyclopamine treatment leads to a strong reduction of Gli1, X-Ptc-1 and X-Ptc-2 expression. Some Gli1 expression remains only in the tailbud (arrowhead in B). Expression of both Gli2 and Gli3 is not affected. Anterior is towards the left. Scale bar: 1.35 mm in A,B; 300 μm in C-L. |