| |
Fig. 5. Colocalization
of a, subunit of L-type
Ca2+ channel and a
subunit of Go protein.
Animal cap of stage 7
(a), stage 8 (bl and
stage 10 Ic) were dissected
and ceffs dissociated
in a Ca2+/Mg2+
free medium before fixing.
Double labeling on
the same cell was carried
out as described in
Experimental procedures.
Left panel: a,
immunolabeling; right
panel: GaD immunolabeling.
Note that the
two different antibodies
labeled the same region
of the plasma membrane.
However some
slight differences may
exit in the colocalization.
Arrowhead in fb)
poinrs to a I positive and
GaD negative domains.
Conversely arrow in (c)
poinrs to aJ negative
and GaD positive
domains_ Bar. 35 pm. |
