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Fig. 3. (Overleaf) Double labelling by in situ hybridization and 5-bromo-29-deoxyuridine (BrdU) antibody. A,B: Stage 40 Xenopus tadpole hindbrain. DMg is expressed strongly in the ventricular zone (A) and BrdU incorporation is mainly seen in the ventricular zone (B). C,D: Metamorphosed froglet (stage 59) forebrain. BrdU-positive cells are clustered around the ventricular zone (D) and distributed similarly as the DMg-positive cells (C). Arrows indicate a cell positive for both DMg and BrdU. Scale bars 5 50 mm in A,B; 25 mm in C,D. |