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Figure 3. Changes in gene expression induced by Hh signaling activation in the froglet blastema at 14 days after amputation. A,D: The Hh-Ag-treated blastema (D) was larger than the control blastema (A). B,C,E,F: In the control blastema, signals for Shh target genes, Ptc-2 and Gli1, were not detected, whereas these genes were strongly re-expressed in the Hh-Ag-treated blastema. G-N: Signals for Gli3 (K) and dHAND (L) were detected in the Hh-Ag-treated blastema and their expression patterns were similar to those in the control blastema (Gli3, arrowheads in G; dHAND, H). Stronger expression of Hoxd13 was observed in the distal region of the Hh-Ag-treated blastema (M), but expression was weak in the control blastema (arrowheads in I). Tbx2 expression was slightly detected in both the control blastema (arrowheads in J) and Hh-Ag-treated blastema (arrowheads in N). All sections are transverse sections, and the anterior side is on the left of all panels. Scale bars = 200 mu m. |
