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Fig. 3. Whole-mount in situ hybridization analysis of XHas1 and XHas2 at tailbud stages. (A) XHas2 expression at stage 28. White arrow: the heart, white arrowhead: branchial arches. (A′) Frontal view of a stage 28 embryo showing the XHas2 expression in the eye (red arrowhead) and in an anterior head region containing the pituitary (yellow arrow). (A′) Histological section of a stage 28 embryo at the eye level showing XHas2 expression in the lens. (B) XMyoD expression in a stage 28 embryo. (C and C′) Horizontal sections of stage 27 embryos hybridized with XSox9 and XHas2 probes, respectively. Red arrows: migrated NCCs. Black arrows: endodermal part of the pharyngeal pouch. |
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fig. 3. Has2 expression at stg. 28 |
