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Fig. 3. XHes2 is atypically regulated by XNgnr1 and Notch. (A) Real-time RT-PCR analysis of XHes2 expression in animal cap assays (equivalent stage=14/15) following XNgnr1 or Notch ICD overexpression. N-tubulin, ESR7 and ESR1 were used as controls for XNgnr1 and Notch ICD activities. Relative values are given as mean±s.e.m. of two (ESR1) or three (XHes2, ESR7, N-tubulin) independent experiments. (B-E) In situ hybridization analysis of XHes2 expression following injection of XNgnr1 (B,C) or Notch ICD (D,E) RNA. (B,C) XHes2 transcription is inhibited within its endogenous domains but activated ectopically within ectoderm and neuroectoderm (white arrowheads) (100%, n=12). (D,E) A weak ectopic expression of XHes2 is observed only along the lateral border of the neural plate (white arrowheads) (75%, n=12). Images correspond to dorsal views with anterior downwards. (C,E) Higher magnifications of areas shown in B,D. Abbreviations: is, injected side; st., developmental stage. |
