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hes5.9xenopus dorso-lateral marginal zone 

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Experiment details for hes5.9

Genome-wide identification of Wnt/β-catenin transcriptional targets during Xenopus gastrulation.

Genome-wide identification of Wnt/β-catenin transcriptional targets during Xenopus gastrulation.

Gene Clone Species Stages Anatomy
hes5.9.L laevis NF stage 11.5 to NF stage 12 dorso-lateral marginal zone

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  Fig. 4. In situ hybridization shows that the expression patterns of many characterized and candidate Wnt/β-catenin direct target genes resemble wnt8 expression. Genes are grouped based on functional role. (A) Expression of wnt8 at different developmental stages. (B) Expression of a few characterized direct target genes of Wnt/β-catenin at different developmental stages. (C) Expression of selected candidate direct target genes. Stages 11.5-12 and 12.5-13 are blastopore view with dorsal up. Neurula stage is dorsal view with anterior up.

Gene Clone Species Stages Anatomy
hes5.9.L laevis NF stage 11.5 dorso-lateral marginal zone

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  Fig. 5. Expression of Wnt/β-catenin target genes is reduced by dkk-injection. (A, C, E) Normalized counts from RNAseq data comparing uninjected control (UC) and dkk-injected embryos for selected known (A) and candidate (C, E) target genes at stage 11.5 (mid-gastrula). (B, D, F) In situ hybridization showing expression pattern of known (B) and candidate (D, F) target genes. Embryos were injected in the right (asterisk) 2 blastomeres at the 4-cell stage with a total of 100 pg dkk. Blue box indicates that embryo was cleared in either benzyl benzoate:benzoic acid (BB:BA) or benzyl benzoate alone (BB; to reduce transparency and visualize the archenteron). Blastopore view with dorsal up, except pnhd is dorsal view with anterior up.

Gene Clone Species Stages Anatomy
hes5.9.L laevis NF stage 11.5 to NF stage 12 dorso-lateral marginal zone

  hes5.9 (hairy and enhancer of split 5, gene 9) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 11.5-12, blastopore view, dorsal up.