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FIG. 6. KRPs colocalise with ER in the cortical region. (a– d) Double immunofluorescence of eggs fixed during the cortical rotation with
anti-tubulin (a, c) and anti-LAGSE (b, d). Anti-LAGSE showed a carpet of superficial cortical staining (a, b) as well as a general codistribution
with the more deeply bundled microtubules of the subcortical vegetal microtubule array (c, d). (e– h) Double immunofluorescence with
anti-LAGSE and anti-BiP to reveal the ER. The distribution of ER at cortical (e) and subcortical (f) levels was similar to that of anti-LAGSE.
Superimposition of images taken both levels (g, h) at high magnification, with the ER outlined in red by application of an edge-finding
function to the anti-BiP image and anti-LAGSE in green, confirmed that the dots of anti-LAGSE overlay parts of the ER network (arrows). |
