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Gene/Clone	Species	Stage	Anatomy Item	Experimenter
irx2	xenopus		dorso-lateral

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Experiment details for irx2

foxD5 plays a critical upstream role in regulating neural ectodermal fate and the onset of neural differentiation.

Gene	Clone	Species	Stages	Anatomy
irx2		xenopus	NF stage 14	dorso-lateral

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Fig. 3. foxD5 mRNA injected into D1.1 lineages affects all 11 NE genes. (A) foxD5-expressing cells (red nuclei) showed increased expression of gem at both gastrulation (left) and neural plate (right) stages. For gastrula, small arrow denotes blastopore lip and inset shows cells at a higher magnification. Dorsal views with anterior to the bottom. sox2 (B) and sox3 (C) were weakly reduced (small arrows) compared to adjacent NE cells () at gastrula stages (dorsal views with anterior to the bottom). This effect waned by neural plate stages, when their expression domains were expanded on the injected side (black lines); anterior views with dorsal to the top. (D) foxD5-expressing cells showed reduced sox11 expression (small arrows) compared to adjacent NE cells () at early gastrula (dorsal view with anterior to the bottom), but later (by st12) showed highly increased expression in both the neural plate (above white arrows) and in the adjacent ectoderm (red asterisk). Black bar indicates the expanded sox11 domain on the injected side. Anterior view with dorsal to the top. (E) soxD expression was unaffected at early gastrulation (dorsal view with anterior to the bottom, and top inset), began to be reduced (small arrows) compared to adjacent NE cells () by stage 12 (bottom inset). The reduced phenotype was maintained at neural plate (anterior-lateral view) stages; higher magnification shows reduced soxD in foxD5-expressing cells (red nuclei) compared to adjacent NE cells (). For zic2 (G), foxD5-expressing cells (dark blue) showed increased expression at both gastrula (bracket and inset) and neural plate stages (arrow depicts ectopic expression along the midline). Both are dorsal views with anterior to the bottom. zic1 (F) and zic3 (H) showed reduced expression in foxD5-expressing cells (red nuclei) compared to adjacent NE cells () at gastrulation. Gaps in their expression (large arrows) coinciding with foxD5-expressing cells (red nuclei) also were observed at neural plate stages. Xiro1 (I), Xiro2 (J) and Xiro3 (K) were repressed (arrows) at both gastrulation (not shown) and neural plate stages. For A–K, the percentage of embryos affected and sample sizes are indicated. (L) These results were confirmed for several of the NE genes by RT-PCR comparing uninjected (Un) and foxD5-injected ACs. H4 is internal control.