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Supplemental Figure S4. JNK inhibitor efficiency in Xenopus. Analysis of SP600125 activity by immunofluorescence for p-c-Jun in non-injected (A, B) or with constitutively active-jnk mRNA-injected animal caps (C, D) treated from stage 11 to 15 with DMSO (A, C) or 0,5μg.ml-1 SP600125 (B, D). Nuclei were stained with Hoechst; red arrowheads: examples of nuclear co-localization between Hoechst and p-c-Jun staining; scale bar, 20 μm. (E) Quantification of the number of cells with a positive nuclear p-c- Jun staining / total number of cells. Mann-Whitney test p values and the total of cells counted (numbers in brackets) are indicated; the values are normalized to NI + DMSO; black bars, DMSO treated animal caps; grey bars, SP600125 treated animal caps. |
