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Fig. 2. Katnal2 localizes to epidermal cilia, basal bodies, and centrioles. A-B) Katnal2 rabbit antibody staining (green) of X. tropicalis multiciliated cells of the epidermis, co-stained with DAPI (nuclei, blue) and acetylated α-tubulin (Ac-α-tubulin; cilia, magenta). B) High-magnification image from the specimen shown in A. C) Katnal2 antibody staining (green) with Centrin4-CFP expression (magenta). D-H) KATNAL2-GFP (green) in the embryonic epidermis of X. laevis, co-stained for Centrin4-RFP (basal bodies/centrioles, magenta) and Ac-α-tubulin (cilia, blue). D-E) KATNAL2-GFP localizes to basal bodies in multiciliated cells. (E is close-up of D) F-H) KATNAL2-GFP localization in non-ciliated epidermal cells. G) KATNAL2-GFP signal in centrioles. H) KATNAL2-GFP signal in the midbody of dividing cells. |
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Fig. S2. Validation of Katnal2 rabbit antibody. A-B) X. tropicalis embryonic epidermis stained for Katnal2 (green) and acetylated α-tubulin (Ac-α-tubulin, cilia, magenta) in the presence (A) or absence (B) of Katnal2 primary rabbit antibody. |
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Fig. 3. Katnal2 localizes to mitotic spindles and ciliary axonemes in the tadpole. A-F) Katnal2 rabbit antibody staining (green) in stage 45 X. tropicalis tadpoles, highlighting staining in ciliated and neural tissues. A) Low magnification view of Katnal2 staining in the cephalic region of the tadpole. B) Telencephalic (tel) region of the brain, showing expression throughout and especially within the first ventricle. B’) Transverse section through the telencephalon. B”) High-magnification image from specimen shown in B’, showing Katnal2 localization to a mitotic spindle. C) Olfactory epithelium (olf) stained for Katnal2 (green), poly-glutamylation (cilia, magenta) and DAPI (nuclei, blue). C’-C”’) High-magnification image of the specimen in C, showing localization of Katnal2 (green) to ciliary axonemes (poly-glutamylation, magenta). D) Eye. E) Multiciliated cells of the roof of the fourth ventricle of the rhombencephalon (rhomb). F) Katnal2 is expressed throughout the kidney. Abbreviations: tel = telecephalon; olf = olfactory epithelium; rhomb = rhombencephalon. |
