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Figure 4. In situ hybridization of xIGFBP-rP10. Positions of sections are indicated by white dotted lines and letters mark corresponding panels. A,B: Sagittal sections. A: Stage (st.) 4. B: St. 10.5; black arrowheads mark dorsal lip; insert: vegetal view. C,D: St. 15. C: Anterior view. D: Dorsal view, anterior to the left; insert: transverse section. E,F: St. 21. E: Anterior view. F: Dorsal view, anterior to the left. G-I: St. 24. G: Lateral view; insert: vegetal view of head. H: Transverse section. I: Crude horizontal section at the level of the notochord. J-M: St. 30. J: Lateral view; insert: vegetal view of head. K-M: Transverse sections. N,O: St. 35; lateral view. O: Magnification of head region. P,Q: St. 40; lateral view. O: Magnification of head region; reversed saturation image of P, to visualize pattern of strongly stained areas. cg, cement gland; cnh, chordoneural hinge; df, dorsal fin; fp, floor plate; hm, head mesenchyme; no, notochord; otv, otic vesicle; so, somite; vf, ventral fin; VII and IX/X, cranial ganglia. |
