| Search Criteria |
| Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
|---|---|---|---|---|
| kcnq1 | xenopus | blastomere [+] |
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Experiment details for kcnq1
Rab GTPases are required for early orientation of the left-right axis in Xenopus.Rab GTPases are required for early orientation of the left-right axis in Xenopus.
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| Fig. 6 DNRab11 alters asymmetric ion transporter localization at the 4-cell stage. (A) 4-cell embryos were oriented, sectioned, and immunostained for ductin protein. Biases in expression were quantified by identifying the strongest expressing blastomere(s). The control embryo shown (i) has the strongest expression in the ventral right cell, verified with ImageJ thresholding tools (ii). The embryo injected with DNRab11 shown (iii) has the strongest expression in the dorsal left cell, verified with ImageJ thresholding tools (iv). (B) A total of 110 control and 45 DNRab11-injected embryos were quantitatively analyzed. In controls, the most common blastomere scored as having the highest expression of ductin was the ventral right; ventral right localization was decreased in DNRab11 injected embryos, although this decrease did not reach statistical significance. Dorsal left expression was rare in control embryos, and the incidence of dorsal left expression was significantly higher in DNRab11-injected embryos (*p < 0.05). (C) The same protocol was used for identifying biases in the expression of KCNQ1. The control embryo shown (i) has the strongest expression of KCNQ1 in the ventral right cell, verified with ImageJ thresholding tools (ii). The embryo injected with DNRab11 shown (iii) has the strongest expression in the dorsal left cell, verified with ImageJ thresholding tools (iv). (D) A total of 85 control and 40 DNRab11-injected embryos were quantitatively analyzed. In controls, the most common blastomere scored as having the highest expression of KCNQ1 was the ventral right; ventral right localization was decreased in DNRab11 injected embryos, although this decrease did not reach statistical significance. Dorsal left expression was relatively rare in control embryos, and the incidence of dorsal left expression was significantly higher in DNRab11-injected embryos (*p < 0.05). | |||||||||||
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| kcnq1 (potassium voltage-gated channel, KQT-like subfamily, member 1) gene expression in Xenopus laevis embryos, NF stage 3, as assayed by immunohistochemistry, ventral side up. |