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Fig. 5. Immunohistochemistry of xLSD1. Sections were prepared from the body skin (A) and the small intestine (B) of the tadpoles
at stages 54 (A1–5, B1–5) and 62 (A6–10, B6–10), and subjected to staining of H&E (A1, A6), M & P (B1, B6), Hoechst 33342 (blue
color: A2, A7, B2, B7), and xLSD1 (green color: A3, A8, B3, B8). A4, A9, B4, and B9 are merged photos of Hoechst and xLSD1
photos. A5, A10 B5, and B10 represent negative stains. (A) Body skin. xLSD1 was localized in the larval basal cells (lb) at stage 54,
but not in apical (ap) and skein cells (sk) (A3). Both adult basal cells (ab) and granular cells (gr) were positive for xLSD1 at stage 62
(A8). The white dotted lines indicate the site of basement membrane. cl, collagen layer. f, fibroblasts. (B) Small intestine. Some larval
epithelial cells (le) and muscle cells (m) are xLSD1-positive at stage 54 (B3). xLSD1 was detected in larval epithelial cells and in few
adult epithelial cells at stage 62 (B8). Orange and white dash lines indicate the region of larval and adult epithelium, respectively.
The white dotted line in B6 shows the site of the basement membrane. Connective tissue cells (ct) were xLSD1-positive.
Immunohistochemistry experiments were carried out three times, which all gave similar patterns as shown here. Scale bars, 50 μm. |
