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Figure S4 (related to Figure 6). Sequence analyses of Kdm2a/b, their expression
patterns in Xenopus early embryos, and their effects on embryonic development.
(A) Kdm2a/b in Xenopus and KDM2A/B in human have the same function domains,
which are highly conserved.
(B) Sequence similarity analysis.
(C and D) The spatial expression patterns of kdm2a (C) and kdm2b (D) in cleavage,
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blastula and gastrula embryos. In each panel, the animal pole of an embryo was at the
top, except the st.12 embryos for which vegetal view was shown. d, dorsal view; v,
ventral view; bl: blastopore lip; yo: yolk plug.
(E) The numbers and percentages of defect embryos among the total number of
embryos that were injected with different doses of Kdm2aMO or Kdm2bMO,
showing the effect of knockdown of Kdm2a/b on body axis development.
(F) Rescue of Kdm2a/b morphants with adding back their respective mRNAs to
embryos, as shown by the numbers and percentages of defect and rescued embryos.
(G) Inhibitory effect of Kdm2a/b on the activity of β-CatδN, as indicated by numbers
and percentages of embryos with double axes and single axis.
(H) The numbers and percentages of embryos with double axes and single axis
indicated that Kdm2a/b did not display significant influence on the partial secondary
axis formation induced by VP16-Tcf7l1(295-441).
(I) The majority of the embryos showed complete ventralization in either UV treated
embryos or in UV treated embryos with subsequent injection of Kdm2a or 2b
morpholino.
(J and K) UV treatment led to inhibition of sia (J) and xnr3 (K) expression, which was
not rescued by Kdm2a/b knockdown in most of the embryos.
(L) Knockdown or overexpression of Kdm2a/b generated no significant effect on cell
adhesion, as shown by animal cap disaggregation/reaggregation assays. The animal
caps injected with ctrlMO and Cdh(1-724) mRNA were used as controls. |
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kdm2a (lysine (K)-specific demethylase 2A) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 3, lateral view, animal up. |
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kdm2a (lysine (K)-specific demethylase 2A) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 10.5, lateral-vegetal view, animal up. |
