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krt12.4xenopus neural plate border [+] 

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Experiment details for krt12.4

Distinct intracellular Ca(2+) dynamics regulate apical constriction and differentially contribute to neural tube closure.

Distinct intracellular Ca2+ dynamics regulate apical constriction and differentially contribute to neural tube closure.

Gene Clone Species Stages Anatomy
krt12.4.L laevis NF stage 16 neural plate border

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  Fig. 1. Intracellular Ca2+ is required for Xenopus NTC. (A) Dorsal views of embryos at stage 16 treated with DMSO, 25 μM 2APB and 200 μM nifedipine. Dashed lines indicate the outlines of the NP. (B) The mean width of the NP in embryos, as measured by visualizing the expression of the pan-neural marker gene Sox2. Error bars depict s.e.m. The number of embryos examined is shown on each bar. **P<0.01 and ***P<0.001 compared with the DMSO-treated control; two-sided Welch’s t-test. (C) Transverse sections of stage-16 embryos stained with phalloidin (top) and outlines of neural tissues and cells (bottom). Scale bars: 100 μm. (D) Apical width of stage- 16 embryos. Black line indicates the median value. **P<0.01 and ***P<0.001, two-sided Mann–Whitney U-test; n=54 cells, nine embryos (DMSO); 97 cells, 12 embryos (2APB); 88 cells, 12 embryos (nifedipine). (E) In situ hybridization analysis of inhibitor-treated embryos. Dorsal views showing the expression of Sox2, a pan-neural marker (top), N-tubulin (tubb2b), which marks differentiated neurons (middle), and Epidermal keratin (Epi. keratin), an epidermal marker (bottom). Anterior is to the top. The expression patterns were similar in inhibitor-treated embryos and DMSOtreated controls, but the expression domains were wider in the inhibitor-treated embryos because of delayed NTC.