| Search Criteria |
| Gene/Clone | Species | Stage | Anatomy Item | Experimenter |
|---|---|---|---|---|
| lama1 | xenopus | stomodeal-hypophyseal primordium [+] |
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Experiment details for lama1
Dickinson AJ and Sive HL (2009) Assay
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| Fig. 7. Laminin persists in the primary mouth region when Frzb-1/Crescent are depleted or when wnt-8 is overexpressed. Sagittal sections (anterior to the left) assayed at stage 35-37, in 2-3 independent experiments. Laminin is immunolabed green, nuclear propidium iodide, red; cement gland is outlined by a dotted gray line. Panels denoted by primes are tracings of Laminin immunolabeling (green). Bracket indicates the presumptive primary mouth; cg, cement gland. Scale bars: 170 μm. (A) Laminin persistence in frzb-1/crescent morphants is specific. (a) Control morpholino and GFP mRNA results in a normal absence of Laminin in the presumptive primary mouth (100%, n=10). (b) In frzb-1/crescent morphants (also injected with control GFP mRNA), Laminin persists in the primary mouth region (87%, n=10). Note that similar phenotypes were seen in morphants injected without GFP mRNA. (c) Control morpholino and frzb-1 mRNA (200 pg) results in a normal absence of Laminin in the presumptive primary mouth (n=10). (d) Co-injection of frzb-1/crescent morpholinos and frzb-1 mRNA restores the absence of Laminin in 70% of morphants (n=10). (B) Laminin persists in embryos locally depleted of frzb-1/crescent. (a) Schematic of the experimental design. (b) Recipients receiving tissue injected with control morpholino and GFP mRNA have a normal absence of Laminin (100%, n=7). (c) Eighty-nine percent of recipients receiving tissue from frzb-1/crescent morphants have persistent Laminin (n=9). (C) Temporal and spatial overexpression of Wnt-8 results in persistent Laminin. (a) Schematic of the experimental design. (b) Recipients receiving tissue injected with GFP::HSE have a normal absence of Laminin (91%, n=11). (c) Seventy-five percent of recipients receiving tissue from embryos injected with GFP::HSE::wnt-8 have persistent Laminin (n=12). (D) Wnt signaling regulates the expression of basement membrane components. Schematic depicts experimental design. Results are an average of two independent experiments. (a) Increased frzb-1 results in fibronectin mRNA that is 45% of the control level, laminin-γ1 mRNA that is 48% of the control level andβ 1-integrin that is 112% of the control level (n=20). (b) Temporally increased wnt-8 results in fibronectin mRNA that is 232% of the control level, laminin-γ1 mRNA that is 170% of the control level and β1-integrin mRNA that is 93% of the control levels (n=20). |