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Fig. 1. Lef1 depletion affects expression of genes in the ventral and dorsal mesoderm. In situ hybridization for X. tropicalis lef1 RNA at stage 10.5 (A) shows high levels of expression in the lateral and ventral marginal zone, low expression in the ectoderm and in the dorsal marginal zone (dorsal side up). Expression of marker genes in gastrula stage embryos, stage 10.5 (B), dorsal side up. Lef1 depletion does not alter the expression of brachyury, wnt8 or myf5. Msr/APJ expression is down- regulated by lef1 depletion whereas nodal related 3 (nr-3) expression is upregulated. Co-injection of human LEF1 RNA rescues the expression of msr/APJ and partially downregulates nr-3 expresssion. |
