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Fig. 1. Scattered cells displaying the typical features of endocrine cells (arrows) are immunoreactive for leptin in the oxyntic glands of O. mykiss.
Bar¼20 lm.
Fig. 2. Pair of consecutive sections of the trout stomach immunostained for leptin. (A) The immunostaining appears in the enteric nervous system.
(B) No immunoreactivity is seen after absorption of the antiserum with 20 lgml 1 of the leptin fragment [137–156]. Bar¼40 lm.
Fig. 3. Panoramic view of the Xenopus oxyntic epithelium immunostained for leptin. The immunoreactivity appears specifically in the oxyntic-peptic
cells. Bar¼40 lm.
Fig. 4. Leptin-immunoreactive fibers in the myenteric plexus of Xenopus. B ar¼20 lm.
Fig. 5. Immunocytochemical detection of leptin in the stomach of the snake. Positive cells are seen in the oxyntic glands and a very strong immunostain
is also found in the enteric nervous system. Bar¼20 lm.
Fig. 6. Two serial sections of the snake stomach showing colocalization of leptin (A) and VIP (B) immunoreactivity in the enteric nervous system.
Bar¼40 lm. |