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Fig. 1. Spatial pattern of Xgalectin-VIa (A,B,C,J,K,L,M,N,O), XCG (D,E,F,Q) and XAG-1 (G,H,I,P) expression by whole-mount in situ ybridization. (A,D,G) Anterior views of the early neurula. Dorsal side is up. (B,E,H) Anterior views of the late neurula. (C,F,I) Lateral views of the tailbud. Xgalectin-VIa was expressed in a highly specific pattern in the cement gland region. (Jâ L) Spatial pattern of Xgalectin-VIa expression at late-tailbud (J) and tadpole (K,L) stages. CG, cement gland; eye, eye vesicle; OV, otic vesicle. (M,N) Histological section of (l). Sectioned regions are indicated in (l). (Oâ Q) Comparison of Xgalectin-VIa expression patterns (O) with those of XAG-1 (P) and XCG (Q) in the anterior region of tadpole-stage embryos. Xgalectin-VIa was expressed in hatching gland (HG; indicated with arrowhead), as observed for XAG-1. |